Fig. 1. Effects of Ang II on beclin-1 protein assayed using western blot. A high dose of Ang II (10⁻⁶ M) increases beclin-1 protein expression at 6 hours (P<0.05) and both 10⁻⁷ M and 10⁻⁶ M of Ang II further increases beclin-1 expression at 12 hours in a dose-dependent manner (P<0.01). Thereafter, beclin-1 protein expression decreases to below the basal level (A). Data on the densitometric analysis of beclin-1/β-tubulin ratio are presented as mean ± SD (n = 3; ^*P<0.05 and ^**P<0.01) (B) Control (100%); compared to conditions without Ang II.